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map short reads to reference fails (Read 934 times)
Feb 10th, 2022 at 12:31am
catherine.reeb   Ex Member

 
Hye I try to reproduce a "mapping to reference" with a ion torrent run, that worked with another program, in order to use only Ugene now. But I cannot get any results, the message is "The short reads can't be mapped to the reference sequence!"

I use .fasq as short reads input
a .fasta as reference

Thanks for help
 
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Reply #1 - Feb 10th, 2022 at 9:31am

Yuliya Algaer   Offline
YaBB Administrator

Posts: 124
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Hi,

Could you please share your FASTA and FASTQ files with us so we can check?

Thank you,
Yuliya
 
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Reply #2 - Feb 10th, 2022 at 4:41pm
catherine.reeb   Ex Member

 
of course,

I tried to post a dropbox link for the fasq (> 250kb) but it does not work because I did not post 5 messages...may I send it via another way ?

and attached the fasta reference
it is a very short marker :chloroplast psba-trnH
... my attachment is too large 846 ko...

Thanks a lot
 
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Reply #3 - Feb 10th, 2022 at 4:51pm

Yuliya Algaer   Offline
YaBB Administrator

Posts: 124
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Please try to send it via e-mail: ugene@unipro.ru
 
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