UGENE Forum - Error with Classify Sequences with DIAMOND element

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Error with Classify Sequences with DIAMOND element (Read 2541 times)

Jan 21^st, 2021 at 3:32pm

zachary33 Offline
YaBB Newbies

Posts: 3

I am using the "Classify Sequences with DIAMOND" element in a workflow for De Novo Assembly of Nanopore reads.

When I run the workflow, it returns the error "Invalid genetic code: 1" under the "Classify Sequences with DIAMOND" element.

What does this mean?

Is there another DIAMOND database I should use? Or could it be another issue upstream in the workflow/pipeline?

I am using the default database (UniRef50) and have also tried using my own DIAMOND database, but the same error occurs.

Thank you for your advice.

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Reply #1 - Jan 22^nd, 2021 at 3:33pm

Dmitrii Sukhomlinov Offline
YaBB Administrator
Russia

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Posts: 78

Hello,

First of all, make sure, that the "DIAMOND" element input port is set to the correct output of SPAdes (see the attached picture).

Also, make sure, that you use the last UGENE v. 37 (there were some problems with diamond which were fixed).

If both points are OK, please, share your data with me if it's possible and I will try to reproduce the problem.

Screenshot_1_001.jpg (241 KB | 287 )

Best regards,
Dmitrii Sukhomlinov,
The UGENE team.

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Reply #2 - Apr 2^nd, 2021 at 10:55am

Bobby Rencher Offline
YaBB Newbies

Posts: 4

I have joined this community and look forward to seeking more.

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