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https://forum.ugene.net/forum/YaBB.pl General Category >> Help and How-to >> Error with Classify Sequences with DIAMOND element https://forum.ugene.net/forum/YaBB.pl?num=1611217938 Message started by zachary33 on Jan 21^st, 2021 at 3:32pm

Title: Error with Classify Sequences with DIAMOND element
Post by zachary33 on Jan 21^st, 2021 at 3:32pm

I am using the "Classify Sequences with DIAMOND" element in a workflow for De Novo Assembly of Nanopore reads.

When I run the workflow, it returns the error "Invalid genetic code: 1" under the "Classify Sequences with DIAMOND" element.

What does this mean?

Is there another DIAMOND database I should use? Or could it be another issue upstream in the workflow/pipeline?

I am using the default database (UniRef50) and have also tried using my own DIAMOND database, but the same error occurs.

Thank you for your advice.

Title: Re: Error with Classify Sequences with DIAMOND element
Post by Dmitrii Sukhomlinov on Jan 22^nd, 2021 at 3:33pm

Hello,

First of all, make sure, that the "DIAMOND" element input port is set to the correct output of SPAdes (see the attached picture).

Also, make sure, that you use the last UGENE v. 37 (there were some problems with diamond which were fixed).

If both points are OK, please, share your data with me if it's possible and I will try to reproduce the problem.

Screenshot_1_001.jpg (241 KB | 277 )

Title: Re: Error with Classify Sequences with DIAMOND element
Post by Bobby Rencher on Apr 2^nd, 2021 at 10:55am

I have joined this community and look forward to seeking more.