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Assembly paired end reads (Read 1119 times)
Dec 18th, 2020 at 3:06am

joseluis   Offline
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Hello. I am new at EUGENE. I want to know how to assembly paired end reads in fastq format using UGENE. Thank you very much.
 
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Reply #1 - Dec 18th, 2020 at 12:38pm

Dmitrii Sukhomlinov   Offline
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Hello

Open UGENE and click "Tools -> NGS Data Analysis -> Map reads to reference...". The corresponding dialog will be opened. You can see here several tools for the reads mapping (Mapping tool), most of them are supposed to map pair-end reads (the "Library" combo box). You may click on the "Help" button to see configuration details (pay attention, that there are separate "Help" pages for different tools).
 

Best regards,
Dmitrii Sukhomlinov,
UGENE team.
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Reply #2 - Dec 18th, 2020 at 1:58pm

joseluis   Offline
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Thanks for your answer, but I want to perform a DE NOVO assembly, without reference. How can I do it?
 
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