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https://forum.ugene.net/forum/YaBB.pl General Category >> Help and How-to >> Assembly paired end reads https://forum.ugene.net/forum/YaBB.pl?num=1608235613 Message started by joselui on Dec 18^th, 2020 at 3:06am

Title: Assembly paired end reads
Post by joselui on Dec 18^th, 2020 at 3:06am

Hello. I am new at EUGENE. I want to know how to assembly paired end reads in fastq format using UGENE. Thank you very much.

Title: Re: Assembly paired end reads
Post by Dmitrii Sukhomlinov on Dec 18^th, 2020 at 12:38pm

Hello

Open UGENE and click "Tools -> NGS Data Analysis -> Map reads to reference...". The corresponding dialog will be opened. You can see here several tools for the reads mapping (Mapping tool), most of them are supposed to map pair-end reads (the "Library" combo box). You may click on the "Help" button to see configuration details (pay attention, that there are separate "Help" pages for different tools).

Title: Re: Assembly paired end reads
Post by joselui on Dec 18^th, 2020 at 1:58pm

Thanks for your answer, but I want to perform a DE NOVO assembly, without reference. How can I do it?