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Primer annotation (Read 6565 times)
Jun 11th, 2014 at 2:25am

lbroetto   Offline
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Hi, how can I insert a primer forward and reverse especific  feature (I mean the arrow direction). Because I was able to add only forward primer arrow to my sequence.

Thanks.
« Last Edit: Jun 11th, 2014 at 4:17am by lbroetto »  
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Reply #1 - Jun 11th, 2014 at 9:55am

Olga Golosova   Offline
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Hello, do you mean a manual creation of a primer annotation in UGENE?

If yes, then there is a button with a curved arrow near the "Location" field in the dialog of the annotation creation. For more details see:
http://ugene.unipro.ru/documentation/manual/sequence_view/annotations_manipulati...

If no, then please describe in more details how you insert the primer.
 
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Reply #2 - Jun 12th, 2014 at 2:08am

lbroetto   Offline
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Exactly, but I have trouble adding different symbols (arrows) to differentiate forward and reverse primers. I have found only forward arrow symbol to add. So the forward and reverse primers look like forward only.

Thanks anyway.
 
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Reply #3 - Jun 12th, 2014 at 2:38am

lbroetto   Offline
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Sorry Olga, I have used an old version of Ugene. Now I have installed the last version and it works.
 
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Reply #4 - Jun 15th, 2014 at 2:19pm

Olga Golosova   Offline
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Great! Thanks for informing me Smiley
 
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