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https://forum.ugene.net/forum/YaBB.pl General Category >> Help and How-to >> Primer annotation https://forum.ugene.net/forum/YaBB.pl?num=1402428332 Message started by lbroetto on Jun 11^th, 2014 at 2:25am

Title: Primer annotation
Post by lbroetto on Jun 11^th, 2014 at 2:25am

Hi, how can I insert a primer forward and reverse especific feature (I mean the arrow direction). Because I was able to add only forward primer arrow to my sequence.

Thanks.

Title: Re: Primer annotation
Post by Olga Golosova on Jun 11^th, 2014 at 9:55am

Hello, do you mean a manual creation of a primer annotation in UGENE?

If yes, then there is a button with a curved arrow near the "Location" field in the dialog of the annotation creation. For more details see:
http://ugene.unipro.ru/documentation/manual/sequence_view/annotations_manipulations/create_a_new_annotation.html

If no, then please describe in more details how you insert the primer.

Title: Re: Primer annotation
Post by lbroetto on Jun 12^th, 2014 at 2:08am

Exactly, but I have trouble adding different symbols (arrows) to differentiate forward and reverse primers. I have found only forward arrow symbol to add. So the forward and reverse primers look like forward only.

Thanks anyway.

Title: Re: Primer annotation
Post by lbroetto on Jun 12^th, 2014 at 2:38am

Sorry Olga, I have used an old version of Ugene. Now I have installed the last version and it works.

Title: Re: Primer annotation
Post by Olga Golosova on Jun 15^th, 2014 at 2:19pm

Great! Thanks for informing me :)