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Read assembly with CAP3 (Read 3192 times)

Jan 5^th, 2022 at 3:31am

Mark Offline
YaBB Newbies

Posts: 1

Hello,

I was hoping to combine a forward and reverse read into a config and was using the tools->sanger data analysis->reads de novo assembly (with CAP3). When I try to combine the two reads I keep getting an error message however "File doesn't contain any msa objects". Does anyone know why this keeps occuring?

Any help would be greatly apprecaited!
Mark

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Reply #1 - Jan 7^th, 2022 at 5:23pm

catherine.reeb Ex Member

Hye Mark
Does your cap3 run ?
I got a sort of similar problem, the path of external tools was done well define, U add to reset it in the settings option (just clock on the button to indicate the folder where tools are stored)
cat

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Reply #2 - Jan 8^th, 2022 at 8:41am

Yuliya Algaer Offline
YaBB Administrator

Posts: 124

Hi Mark,
Catherine is right. Could you please check that the tools are set up correctly ("Settings"->"Preferences"->"External Tools")?

They should already be pre-configured if you use our version of UGENE. Do you use some custom-built versions?

Thank you,
Yuliya

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