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Merge multiple sequences (Read 1227 times)
Dec 10th, 2021 at 11:36am

yusuke1   Offline
YaBB Newbies

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I thank the developer every time I use UGENE.
I want the feature to merge multiple sequences, and it would be best if the overlapping parts of two sequences could be converted to a consensus sequence if they do not match.
This would be useful for merging Sanger sequences read from the 3' end and 5' end sides.

Thank you,
 
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Reply #1 - Dec 13th, 2021 at 11:55am

Dmitrii Sukhomlinov   Offline
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Russia

Gender: male
Posts: 62
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Hello,

Could you, please, detail this a bit. How this feature differs from the consensus? You may export the consensus from the Sanger Reads Editor as a sequence (see attachment 1), also, you may export the whole Sanger alignment as Multiple alignment with the consensus (see attachment 2). Isn't this fit to you?
 

1_012.png (48 KB | 116 )
1_012.png
2_010.png (33 KB | 104 )
2_010.png

Best regards,
Dmitrii Sukhomlinov,
The UGENE team.
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Reply #2 - Dec 20th, 2021 at 4:14pm

yusuke1   Offline
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Thank you for your kind reply!
I was not aware of this method until now.
It is true that I can merge multiple sequences of the same region of the same individual with the Sanger Reads Editor.

I often sequence a particular region of the same individual from the 5-end and 3-end sides, and it is time-consuming to merge them one by one in the Sanger Reads Editor.

It would be very helpful if I could use Multiple Sequence Alignment Viewer to merge the sequences.

Many thanks,
 
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