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Align to reference? (Read 14274 times)
Aug 23rd, 2013 at 6:28am

Ilya Flyamer   Offline
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How can one align a few sequences to one reference sequence in Ugene? So that those sequences don't get aligned to each other at the same time. It should be easy, but I can't find a way to do that.
 
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Reply #1 - Aug 26th, 2013 at 12:58pm

Yuriy Vaskin   Offline
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Hi there!

In Multiple alignment editor try to use the context menu and "Align sequences to profile with MUSCLE". The reference sequence is the profile.
 
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Reply #2 - Aug 26th, 2013 at 4:11pm

Konstantin Okonechnikov   Offline
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Hi,

another option would be to use BWA SW.

Select menu item "Tools->Align to reference->Align short reads" and then choose BWA SW as alignment algorithm. It is targeted on Sanger-like reads (with length up to several Kbp) and will work fine for long sequences.
 
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Reply #3 - Aug 30th, 2013 at 10:17pm

Ilya Flyamer   Offline
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Yuriy Vaskin wrote on Aug 26th, 2013 at 12:58pm:
Hi there!

In Multiple alignment editor try to use the context menu and "Align sequences to profile with MUSCLE". The reference sequence is the profile.


It can't work with opened file, can it? It asks to open another file with sequences - will it align only them to the reference sequence or how will it work?
 
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Reply #4 - Aug 30th, 2013 at 10:18pm

Ilya Flyamer   Offline
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Posts: 126
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Konstantin Okonechnikov wrote on Aug 26th, 2013 at 4:11pm:
Hi,

another option would be to use BWA SW.

Select menu item "Tools->Align to reference->Align short reads" and then choose BWA SW as alignment algorithm. It is targeted on Sanger-like reads (with length up to several Kbp) and will work fine for long sequences.


Again, it asks to open another files, doesn't it?

Are they just not fully integrated on some reason?
 
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Reply #5 - Sep 9th, 2013 at 12:00pm

Yuriy Vaskin   Offline
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In both cases your sequences must be loaded from a file. Try to export them from the project into a file and then use one of the options to align them.
 
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