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how to join two sequences while preserving their annotations (Read 4240 times)
Jan 14th, 2013 at 3:15pm

randolf   Offline
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Hi,
I'can't find a way to join two sequences while preserving their annotations. The only way I could find to add a sequence to a sequence in the viewer is with "insert subsequence" but there it is only possible to insert a sequence without its annotations.

Thanks for any help!
Thomas
« Last Edit: Jan 14th, 2013 at 6:04pm by randolf »  
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Reply #1 - Jan 14th, 2013 at 8:02pm

Konstantin Okonechnikov   Offline
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Hi Thomas,

there are several ways join sequences preserving annotations.

First option is to export merged sequence to single document.

Select your sequences in Project View and call context menu item Export sequence.
In appeared dialog set your file format to Genbank, set box Export with annotations checked and select radio button Merge sequences.
Detailed documentation of this option can be found here.

Second option is to use Coning In Silico.

Call menu Tools->Cloning->Construct molecule.... Use button From project to add sequences, which you wish to join. Add them to new molecule contents using buttons Add or Add all. After that check option Force "blunt" and omit overhangs. Additionally you can uncheck option Annotate fragments in new molecule.
More documentation on this option can be found here, but I think it's a bit outdated.

 
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Reply #2 - Jan 16th, 2013 at 4:10pm

randolf   Offline
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Hello Konstantin,
Thanks a lot! Cloning the fragments with the second method worked well.
 
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