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General Category >> Help and How-to >> how to join two sequences while preserving their annotations https://forum.ugene.net/forum/YaBB.pl?num=1358151326 Message started by randolf on Jan 14th, 2013 at 3:15pm |
Title: how to join two sequences while preserving their annotations Post by randolf on Jan 14th, 2013 at 3:15pm
Hi,
I'can't find a way to join two sequences while preserving their annotations. The only way I could find to add a sequence to a sequence in the viewer is with "insert subsequence" but there it is only possible to insert a sequence without its annotations. Thanks for any help! Thomas |
Title: Re: how to join two sequences while preserving their annotations Post by Konstantin Okonechnikov on Jan 14th, 2013 at 8:02pm
Hi Thomas,
there are several ways join sequences preserving annotations. First option is to export merged sequence to single document. Select your sequences in Project View and call context menu item Export sequence. In appeared dialog set your file format to Genbank, set box Export with annotations checked and select radio button Merge sequences. Detailed documentation of this option can be found here. Second option is to use Coning In Silico. Call menu Tools->Cloning->Construct molecule.... Use button From project to add sequences, which you wish to join. Add them to new molecule contents using buttons Add or Add all. After that check option Force "blunt" and omit overhangs. Additionally you can uncheck option Annotate fragments in new molecule. More documentation on this option can be found here, but I think it's a bit outdated. |
Title: Re: how to join two sequences while preserving their annotations Post by randolf on Jan 16th, 2013 at 4:10pm
Hello Konstantin,
Thanks a lot! Cloning the fragments with the second method worked well. |
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