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UGENE Forum › General Category › Help and How-to › BLAST output with Workflow designer

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BLAST output with Workflow designer (Read 6868 times)

Apr 16^th, 2012 at 11:03am

kenkl Offline
Junior Member

Posts: 73

Hi there,

I'm using analysing sequences with BLAST in the workflow designer, but could not get the desired output (with blue boxes indicating homologous region). Can give some tips on this?

Thanks!

Kenneth

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Reply #1 - Apr 16^th, 2012 at 2:31pm

German Offline
Full Member

Posts: 118

Kenneth, could you help us a bit, please? We need to know the next information:
1) What blast tool do you use? (is it downloaded from our web site or is it some other version of blast?)
2) What parameters do you set for blast element? (It will be great if you share the uwl file with us.)
3) Could you share please the input files of the scheme if they are not so big?

Thanks for your feedback!

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Reply #2 - Apr 16^th, 2012 at 2:43pm

kenkl Offline
Junior Member

Posts: 73

I'm using blastall in Ubuntu, using the sample data file in UGENE as my input to blast against a formatted database. The workflow is simple and can be easily reproduce:

Read sequence > Local BLAST > Write Sequence (Genbank)

If you still need the files I can send it to you. Thanks!

Kenneth

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Reply #3 - Apr 16^th, 2012 at 3:02pm

German Offline
Full Member

Posts: 118

kenkl wrote on Apr 16^th, 2012 at 2:43pm:

So, your blast tool is not downloaded from our site, but it is from Ubuntu repository. What is the version of your blast tool?

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Reply #4 - Apr 16^th, 2012 at 3:08pm

kenkl Offline
Junior Member

Posts: 73

blast 2.2.24

I had just downloaded the one from your website and it works fine. Thats has to be done with the compatibility right? Just a thought. Thanks!

Kenneth

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