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BLAST output with Workflow designer (Read 6477 times)
Apr 16th, 2012 at 11:03am

kenkl   Offline
Junior Member

Posts: 73
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Hi there,

I'm using analysing sequences with BLAST in the workflow designer, but could not get the desired output (with blue boxes indicating homologous region). Can give some tips on this?

Thanks!

Kenneth
 
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Reply #1 - Apr 16th, 2012 at 2:31pm

German   Offline
Full Member

Posts: 118
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Kenneth, could you help us a bit, please? We need to know the next information:
1) What blast tool do you use? (is it downloaded from our web site or is it some other version of blast?)
2) What parameters do you set for blast element? (It will be great if you share the uwl file with us.)
3) Could you share please the input files of the scheme if they are not so big?

Thanks for your feedback!
 
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Reply #2 - Apr 16th, 2012 at 2:43pm

kenkl   Offline
Junior Member

Posts: 73
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I'm using blastall in Ubuntu, using the sample data file in UGENE as my input to blast against a formatted database. The workflow is simple and can be easily reproduce:

Read sequence > Local BLAST > Write Sequence (Genbank)

If you still need the files I can send it to you. Thanks!

Kenneth
 
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Reply #3 - Apr 16th, 2012 at 3:02pm

German   Offline
Full Member

Posts: 118
***
 
kenkl wrote on Apr 16th, 2012 at 2:43pm:
I'm using blastall in Ubuntu, using the sample data file in UGENE as my input to blast against a formatted database. The workflow is simple and can be easily reproduce:

Read sequence > Local BLAST > Write Sequence (Genbank)

If you still need the files I can send it to you. Thanks!

Kenneth


So, your blast tool is not downloaded from our site, but it is from Ubuntu repository. What is the version of your blast tool?
 
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Reply #4 - Apr 16th, 2012 at 3:08pm

kenkl   Offline
Junior Member

Posts: 73
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blast 2.2.24

I had just downloaded the one from your website and it works fine. Thats has to be done with the compatibility right? Just a thought. Thanks!

Kenneth
 
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