Welcome, Guest. Please Login or Register
UGENE Bulletin Board
  Welcome to our forum.
  HomeHelpSearchLoginRegister  
 
 
Page Index Toggle Pages: 1
Exporting features as alignments (Read 10059 times)
Nov 17th, 2010 at 8:18pm

Agu   Offline
Junior Member
(Argentinian)

Gender: male
Posts: 63
**
 
1- If I export several features as alignments, the sequences I get are the reverse complementaries instead of the originals.

2- Also, I've seen that sometimes you can export the translation of the selected sequences, but that option appears quite seldom. I'm not sure if I'm doing something wrong or if this is a bug.  Undecided

Thanks
 
IP Logged
 
Reply #1 - Nov 18th, 2010 at 1:45pm

Konstantin Okonechnikov   Offline
Global Moderator

Posts: 173
*****
 
Agu wrote on Nov 17th, 2010 at 8:18pm:
1- If I export several features as alignments, the sequences I get are the reverse complementaries instead of the originals.

We'll fix this bug in the next release (1.8.1)

Quote:
2- Also, I've seen that sometimes you can export the translation of the selected sequences, but that option appears quite seldom. I'm not sure if I'm doing something wrong or if this is a bug.  Undecided


This could be a bug. The translation is a standard option of any "Export" dialog. (See the image attached) However this option depends on the alphabet. The option is not visible if the sequence alphabet is detected as Raw or is not set by some reason.

Could you please describe a situation when this is option is not shown? What is the format of the file? Was it obtained from specific database or created by some software? If it is created by UGENE what was the pipeline?


 

pic.PNG (32 KB | )
pic.PNG
IP Logged
 
Reply #2 - Nov 18th, 2010 at 10:50pm

Mikhail Fursov   Offline
YaBB Administrator

Gender: male
Posts: 162
*****
 
Agu wrote on Nov 17th, 2010 at 8:18pm:
1- If I export several features as alignments, the sequences I get are the reverse complementaries instead of the originals.


Fixed.
Still wonder why we didn't have tests for these feature before ...
 

---
UGENE team
IP Logged
 
Reply #3 - Nov 19th, 2010 at 10:32pm

Agu   Offline
Junior Member
(Argentinian)

Gender: male
Posts: 63
**
 
image
 

automatic.png (11 KB | )
automatic.png
IP Logged
 
Reply #4 - Nov 19th, 2010 at 10:33pm

Agu   Offline
Junior Member
(Argentinian)

Gender: male
Posts: 63
**
 
Quote:
Could you please describe a situation when this is option is not shown? What is the format of the file? Was it obtained from specific database or created by some software? If it is created by UGENE what was the pipeline?

Now I figure it out, so I can give a better explanation. My goal was, working with several genbank files in the same display, to select the same feature in the different sequences and to make an alignment. It would be nice to be able to do that for both, DNA and protein.
If I select the different features, I only have the option for a DNA alignment:

...

Now, if I have the Details view activated and select some sequence for both, I get the option for translated alignment:

...

The "random" behavior came when I had some sequence selected in the detailed view. Even hiding  this views and selecting features, I get the option for translation:

...

Then, the problem is that in this last case, Ugene will use the selected features shown in the screen if you choose the DNA alignment, while it will use the "other" selected sequences (hidden or not) when selecting the translation option. This could be solved with an automatic selection in the detail view. I mean, automatically selecting the corresponding sequence in the detail view when selecting a feature in the zoom view. Also, for some situations It would be much faster than having to select a feature and then going to "Select/Sequence around selected annotation".
I can't think of a negative side for that behavior (actually, I believe it would be a nice feature).
Just to make it more clear, after selecting a feature with one click it should look like this:

...


Hope this helps...

 

Detail_view.png (114 KB | )
Detail_view.png
Selected.png (89 KB | )
Selected.png
IP Logged
 
Reply #5 - Nov 22nd, 2010 at 1:13pm

Konstantin Okonechnikov   Offline
Global Moderator

Posts: 173
*****
 
Eitel,
you can select sequence around annotation automatically by using Ctrl + Mouse Click on it. I've just discovered that we don't have this useful feature documented. We will consider having this mode as default.

Looks like having selected both annotations and sequence regions might be a little bit confusing. There is no option to make alignment from the translated sequences of selected annotations, thus only option for selected sequence regions is shown and it's named not very clearly. We will add the same functionality for selected annotations and think on how to improve overall design of this feature.


 
IP Logged
 
Reply #6 - Nov 23rd, 2010 at 5:38pm

Mikhail Fursov   Offline
YaBB Administrator

Gender: male
Posts: 162
*****
 
This could be solved with an automatic selection in the detail view. I mean, automatically selecting the corresponding sequence in the detail view when selecting a feature in the zoom view

Fixed. Now with every annotation selected a sequence region around the annotation is also selected, so you have 2 sets of options: work with annotation selection or with sequence selection independently



My goal was, working with several genbank files in the same display, to select the same feature in the different sequences and to make an alignment. It would be nice to be able to do that for both, DNA and protein.

The feature is implemented too. Thanks for your feedback
 

---
UGENE team
IP Logged
 
Reply #7 - Nov 26th, 2010 at 11:23pm

Agu   Offline
Junior Member
(Argentinian)

Gender: male
Posts: 63
**
 
Great!

Looking forward for the next version.


Agu
 
IP Logged
 
Page Index Toggle Pages: 1