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How to create consensus sequence from forward and reverse chromatogram (Read 13665 times)
Reply #15 - Sep 27th, 2019 at 12:36pm

Bernie   Offline
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Hello,

thank you for the information!
To solve my problem, would it be correct to start in the following way:
"Tools > Sanger data analysis > Map reads to reference...", choose the forward chromatogram as reference and then map forward and reverse chromatogram to that reference?
This way would be a bit sinpler to go.

Bernie
 
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Reply #16 - Sep 27th, 2019 at 12:58pm

Olga Golosova   Offline
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Hello, Bernie.

Quote:
To solve my problem, would it be correct to start in the following way:
"Tools > Sanger data analysis > Map reads to reference...", choose the forward chromatogram as reference and then map forward and reverse chromatogram to that reference?
This way would be a bit sinpler to go.

Yes, this approach can also work, if the reads intersection (i.e. the aligned region) is long enough.
 
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