Welcome, Guest. Please Login or Register
UGENE Bulletin Board
  Welcome to our forum.
  HomeHelpSearchLoginRegister  
 
 
Pages: 1 2 
How to create consensus sequence from forward and reverse chromatogram (Read 16996 times)
Reply #15 - Sep 27th, 2019 at 12:36pm

Bernie   Offline
YaBB Newbies

Gender: male
Posts: 9
*
 
Hello,

thank you for the information!
To solve my problem, would it be correct to start in the following way:
"Tools > Sanger data analysis > Map reads to reference...", choose the forward chromatogram as reference and then map forward and reverse chromatogram to that reference?
This way would be a bit sinpler to go.

Bernie
 
IP Logged
 
Reply #16 - Sep 27th, 2019 at 12:58pm

Olga Golosova   Offline
YaBB Administrator

Posts: 338
*****
 
Hello, Bernie.

Quote:
To solve my problem, would it be correct to start in the following way:
"Tools > Sanger data analysis > Map reads to reference...", choose the forward chromatogram as reference and then map forward and reverse chromatogram to that reference?
This way would be a bit sinpler to go.

Yes, this approach can also work, if the reads intersection (i.e. the aligned region) is long enough.
 
IP Logged
 
Pages: 1 2