Hello. You're OK about the etiquette, don't worry! As I've understood, you're trying to run the "De novo assembly Illumina PE reads" workflow. The last "Assembly reads with SPAdes" step could require a lot of time, but 5 hours is a bit too much. First of all, left the execution for all night - it's possible, that data you need to process is really very time-consuming. It also depends on the processing power of your computer, so is your machine isn't very quick it definitely could be a bit long. If it won't finish at all, could you, please, share your data with me and I will try to figure it out?
Best regards, Dmitrii Sukhomlinov, The UGENE team.
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