LOCUS pSAG1-mCherry 4833 bp 18-MAR-2014 EMBL_ID pSAG1-mCherry 4833 bp CIRCULAR 02-MAR-2014 UNIMARK pSAG1-mCherry annotations pSAG1-mCherry FEATURES Location/Qualifiers source 1..694 /organism="pBlueScript SK-" /mol_type="other DNA" /label="source pBlueScript SK-" misc_feature 1..702 /Source="referenceToDocument: urn:local:.:1370979308487.115" /label="pGRA1-GFP 5' (concatenated sequence 1)" /note="Geneious type: Concatenated sequence" misc_feature 1..694 /Source="referenceToDocument: urn:local:.:1370979308487.116" /label="pBlueScript SK- (modified) extraction 5' to HindIII (concatenated sequence 1)" /note="Geneious type: Concatenated sequence" rep_origin 24..330 /label="f1_origin" misc_feature complement(474..622) /label="lacZ_a" misc_feature 588..610 /label="M13_pUC_fwd_primer" promoter 603..619 /label="M13_forward20_primer" promoter 626..644 /label="T7_promoter" misc_feature 669..685 /label="pBluescriptKS_primer" misc_feature complement(678..702) /created_by="User" /label="SCvectorR" /note="Geneious type: primer_bind_reverse" primer_bind 688..725 /created_by="User" /label="SAG1proF" misc_feature 689..694 /created_by="User" /label="HindIII" /note="Geneious type: restriction_site" misc_feature 695..702 /created_by="User" /label="SwaI" /note="Geneious type: restriction_site" misc_feature 703..1454 /Source="referenceToDocument: urn:local:.:1370979308487.113" /label="SAG1 promoter (concatenated sequence 2)" /note="Geneious type: Concatenated sequence" promoter 703..1454 /created_by="User" /label="SAG1 promoter" misc_feature complement(1430..1469) /created_by="User" /label="SAG1proR" /note="Geneious type: primer_bind_reverse" primer_bind 1455..1474 /created_by="User" /label="mCherryF" CDS 1455..2162 /created_by="User" /label="mCherry" misc_feature 1455..2165 /Source="referenceToDocument: urn:local:.:1370979308487.114" /label="mCherry (concatenated sequence 3)" /note="Geneious type: Concatenated sequence" misc_feature complement(2141..2180) /created_by="User" /label="mCherryR" /note="Geneious type: primer_bind_reverse" misc_feature complement(2141..2162) /created_by="User" /label="mCherry_colonyR" /note="Geneious type: primer_bind_reverse" misc_feature 2163..2165 /created_by="User" /label="Stop" misc_difference 2166..2173 /created_by="User" /label="PacI" primer_bind 2166..2190 /created_by="User" /label="SCvectorF" misc_feature 2166..4833 /Source="referenceToDocument: urn:local:.:1370979308487.111" /label="pGRA1-GFP 3' (concatenated sequence 4)" /note="Geneious type: Concatenated sequence" misc_feature 2166..2593 /Source="referenceToDocument: urn:local:.:1370979308487.117" /label="GRA2-3'UTR (concatenated sequence 1)" /note="Geneious type: Concatenated sequence" misc_feature 2234..2237 /Recognition_pattern="GA^TC" /annotation_group="GATC" /label="DpnI" /note="Geneious type: restriction site" misc_feature complement(2315..2334) /created_by="User" /modified_by="User" /label="GRA2-3'UTR_colonyR" /note="Geneious type: primer_bind_reverse" misc_feature 2422..2425 /Recognition_pattern="C^CGG" /annotation_group="CCGG" /label="HpaII, MspI" /note="Geneious type: restriction site" misc_feature 2460..2465 /Recognition_pattern="CA^TATG" /annotation_group="CATATG" /label="NdeI" /note="Geneious type: restriction site" misc_feature 2588..2593 /Recognition_pattern="G^TCGAC" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" source 2594..4833 /organism="pBlueScript SK-" /mol_type="other DNA" /label="source pBlueScript SK-" misc_feature 2594..4833 /Source="referenceToDocument: urn:local:.:1370979308487.112" /label="pBlueScript SK- (modified) extraction 3' (concatenated sequence 2)" /note="Geneious type: Concatenated sequence" misc_feature complement(2595..2611) /label="pBluescriptSK_primer" misc_feature 2630..2635 /created_by="User" /label="SacI" /note="Geneious type: restriction_site" promoter complement(2647..2666) /label="T3_promoter" promoter complement(2683..2701) /label="M13_reverse_primer" misc_feature complement(2700..2722) /label="M13_pUC_rev_primer" promoter complement(2736..2765) /label="lac_promoter" gene complement(3848..4708) /gene="Ampicillin" /label="Ampicillin" CDS complement(3848..4708) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label="ORF frame 3" promoter complement(4750..4778) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label="AmpR_promoter" ORIGIN 1 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 61 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 121 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 181 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 241 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 301 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 361 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 421 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG 661 GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTATTTAA ATTTTACATC CGTTGCCTTT 721 TCCACGGTCC GTGATTTCAT GTGCGTGCAG CTTCAAAGAC TGGTCGTTGC GACTAATAAG 781 ACTGCAGTGA CAGGTCGAAT GGTGGGCACC TTGCTGATGA CTATCTACTG CAAAGTCTGA 841 GACAAGGAAC GAAACTTCCC ACACGAGGCA TTTGAAACTG ACGGTGTCTA GGTAATATGC 901 ACTGCAAGAC ACGGTACTGG GGCCTCGCTG AATTAGGGGC CGATCTCGTT GCCCTATCAG 961 TGCTCACAGT GCCGCAACGT AACACCAGGG CAGGTTCTTG ACAGTGGCAA CAATGTGCGA 1021 CGGGCGTGTG AACGTTTCGT AGTCATAGCG CTAGCACGTA CCTAGCCACG TGGTCGTGAG 1081 GAGCTTTACC ATGCGTCTAG AAGGTGGATG CGGGACACGC CTTCCTGGCC TTTGGCTCCT 1141 GAGACGCGTG TTCTAACCAC AAAGCTTGAG ACGCGTGTTC CAACCACGCA CCCTGAGACG 1201 CGTGTTCTAA CCACGCACCC TGAGACGCGT GTTCTAACCA CGCACCCTGA GACGCGTGTT 1261 CTAACCACGC ACCCTGAGAC GCGTGTTCTG CCGCACAATG TGCACCTGTA GGAAGCTGTA 1321 GTCACTGCTG ATTCTCGCTG TTCTCGGCAA GGGCTGACGA CCGGAGTACA GTTTTTGTGG 1381 GCAGAGCCGC TGTGCAGCTT TCCGTTGTTC TCGGTTGTGT CACATGTGTC ATTGTCGTGT 1441 AAACACACGG TTGTATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT 1501 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG 1561 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG 1621 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG 1681 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT 1741 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT 1801 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT 1861 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT 1921 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG 1981 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG 2041 GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG 2101 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA 2161 AGTAGTTAAT TAAGACTACG ACGAAAGTGA TGCGCAGGCT GGAAAGCCGC TGAAGGGAGA 2221 AGTCTACAAA GCCGATCAGT GAAAAATGTG TGGGGAGGTG GTCTTGTTGC AGGAATGCAA 2281 TGGTGTTAAG CATCGTGTTC GAATGCAGTG CGTGTATCAG TTGTGCGCGG AAGGACACTG 2341 CTTCAATGTT AAGAACCTGT TTTCTCCGTA GAGAGGACCA AAAGACGATT GCAAAACTGG 2401 TATGTACGCA ATAGCCCAAT GCCGGACGTC AGTTGGTTGT ATGTGACGCT CCCAGATGTC 2461 ATATGCCTTG TGAGTGTGTC TGGGATGCAA GTTTTTGGTG TGCGTTGATT TCGCCAGCTT 2521 ATGACAGTGG CAGACGAATT ATTGACATGA TACAAGGACG CAGAAAGGAA CAAACACCGT 2581 AGTTCCAGTC GACGGATCCA CTAGTTCTAG AGCGGCCGCC ACCGCGGTGG AGCTCCAGCT 2641 TTTGTTCCCT TTAGTGAGGG TTAATTTCGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 2701 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 2761 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 2821 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 2881 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 2941 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3001 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 3061 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 3121 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 3181 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 3241 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 3301 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 3361 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 3421 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 3481 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 3541 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 3601 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 3661 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 3721 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 3781 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 3841 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 3901 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 3961 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 4021 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 4081 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 4141 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 4201 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 4261 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 4321 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 4381 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 4441 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 4501 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 4561 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 4621 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 4681 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 4741 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 4801 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGC //