č!˙AGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGAATTAGCTTCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGATTAATTAACATATGAGCGCTGAAGTGCATACCAATCAGGACCCGCTGGATGAAGTCCACACaAACCAaGATCCaCTCGATACTAGTctggagGTCctgttccagggacctTCGAGCCTGGAAGTTCTGTTCCAGGGGCCCCTGTCGATAACTTCGTATAGTACACATTATACGAAGTTATCTCTCGAGAGCAAAGGAGAGGAGCTGTTCACAGGAGTGGTGCCTATCCTGGTGGAGCTGGATGGAGATGTGAACGGCCACAAGTTCAGCGTGTCTGGAGAGGGAGAAGGAGATGCTACCTATGGCAAGCTGACCCTCAAACTGATCTGCACCACAGGAAAGCTGCCTGTGCCCTGGCCCACCCTGGTGACAACCCTGGGCTATGGACTCCAGTGCTTTGCCAGATACCCTGACCACATGAAGCAGCATGACTTCTTCAAGTCTGCCATGCCTGAGGGCTATGTGCAGGAAAGGACCATCTTCTTTAAGGATGATGGCAACTACAAGACCAGGGCTGAGGTGAAGTTTGAGGGAGACACCCTGGTGAACAGGATTGAGCTGAAGGGCATTGACTTCAAGGAGGATGGAAACATCCTGGGCCACAAACTGGAGTACAACTACAACTCCCATAATGTGTACATCACAGCAGACAAACAGAAGAATGGCATCAAAGCCAACTTCAAGATCAGACACAACATTGAGGATGGAGGGGTGCAACTGGCAGACCACTACCAGCAAAACACCCCCATTGGAGATGGCCCTGTGCTGCTCCCTGACAACCACTACCTGAGCTACCAGAGCAAGCTGTCCAAAGACCCCAATGAGAAGAGGGATCACATGGTCCTCCTGGAATTTGTCACTGCTGCTGGCATCACACATGGCATGGATGAGCTGTACAAGCTCGAGATAACTTCGTATAGTACACATTATACGAAGTTATCCggcctgaacgacatcttcgaggcccagaagatcgagtggcacgaaGTAACTTTAAATAATGCCAATTATTTAAAGTTAAGAGCCGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAAAATCCCGGGCCCGCTATGGCCACAACCTGTTACATTGCACAAGATAAAAATATATCATCACGAACAGTAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGGCAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGTAACTTTAAATAATGCCAATTATTTAAAGTTAAGATCTGATTATAAAGACCACgatggagactataaagatcatgacattgactacaaggatgacgacgacaagAGCGCTTGACTAAATAGTTAATTAACATATGGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACgAACCCTTTGGCaAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAACGGCCACGATGCGTCCGGCGTAGAGGATCTGAAGATCAGC[DEFINITION]: [CDS]: 624..133400'R6K gamma origin//label="R6K gamma origin" - complement(1..377)Region3771 132,164,192,R6K ori2/note="R6K gamma origin" - /label="R6K ori" - 378Site 132,164,192,PacI/label=PacI - 436..443Site436443 132,164,192,NdeI/label=NdeI - 444..449Site444449 132,164,192,AfeIU/note="Isoschizomer with AitI, Aor51HI, Eco47III, and FunI. - /label=AfeI - 450..455Site450455 132,164,192,Eco47III/label=Eco47III - 450..455Site450455 132,164,192, 2 ty1 tag/label="2 ty1 tag" - 456Site 132,164,192,optimized 2xty1$/label="optimized 2xty1" - 456..515Region456515 132,164,192,PreS/label=PreS - 522Site 132,164,192, PreScission/label=PreScission - 522..545Region522545 132,164,192,PreSG/note="extend PreS including flexible linker" - /label=PreS - 546..581Region546581 132,164,192,lox5171/label=lox5171 - 582..615Region582615 132,164,192,mVenus/label=mVenus - 624Site 132,164,192,mVenus, codon-optimised-/label="mVenus, codon-optimised" - 624..1334CDS6241334 132,164,192,AvaI˙/note="Isoschizomer with AcrI, Ama87I, AquI, AspBI, AspCI, AspDI, AvrI, BcoI, Bse15I, BsoBI, BstBAII, BstNSII, Bst7QI, BstSI, BstZ4I, Eco88I, Eco27kI, EspHK29I, NliI, Nli3877I, NmuAI, NspIII, NspDI, NspEI, NspSAI, PlaAI, PunAI, Uba1205II, Uba1436I, Uba144Site13351340 132,164,192,XhoI/label=XhoI - 1335..1340Site13351340 132,164,192,/label= - 1335..1610CDS13351610 132,164,192,lox5171/label=lox5171 - 1341..1374Region13411374 132,164,192,BiotinN/note="15-mer GLNDIFEAQKIEWHE biotin acceptor peptide" - /label=Biotin - 1377Site 132,164,192,Biotin/label=Biotin - 1377..1421Region13771421 132,164,192,_Region/note=rox - 1423..1454Region14231454 132,164,192,T2A/label=T2A - 1455Site 132,164,192,T2A/label=T2A - 1455..1514Region14551514 132,164,192,gb3/label=gb3 - 1517Site 132,164,192,gb3/label=gb3 - 1517..1610Region15171610 132,164,192,neoR/label=neoR - 1611Site 132,164,192,neomycin phosphotransferase IIm/note="G418 and kanamycin resistance (NTP_II marker)" - /label="neomycin phosphotransferase II" - 1611..2414CDS16112414 132,164,192,_Region/note=rox - 2416..2447Region24162447 132,164,192,BglII/label=BglII - 2448..2453Site24482453 132,164,192,3xFLAG/label=3xFLAG - 2454Site 132,164,192,3xFLAGf/note="D Y K D H D G D Y K D H D I D Y K D D D D K" - /label=3xFLAG - 2454..2519CDS24542519 132,164,192,AfeIW/note="Isoschizomer with AitI, Aor51HI, Eco47III, and FunI. - /label=AfeI - 2520..2525Site25202525 132,164,192,Eco47III/label=Eco47III - 2520..2525Site25202525 132,164,192,stop2/note="all-frame stop" - /label=stop - 2526..2536Region25262536 132,164,192,PacI/label=PacI - 2537..2544Site25372544 132,164,192,NdeI/label=NdeI - 2545..2550Site25452550 132,164,192,Flag tagCDS249625190,128,0,AvitagCDS137714210,128,0,AvitagCDS137714210,128,0,