Hi, I am trying to assemble two .fq files obtained with illumina paired ends. This files already have been used to assemble contigs using Shovill of Galaxy, however, when I try to run an assembly with UGene, the pipe stops with the message "Subtask {GenomeAssemblyTask} is failed: Subtask {SPAdes tool} is failed: SPAdes tool exited with code 245". Can you give any advice, please?

Ensure that your .fq files are clean and do not have any errors in the data (e.g., quality control issues). You could run a QC step using tools like FastQC to check the quality of your reads before attempting assembly.