Hello,

for cloning purposes, I would like to simulate a restriction digestion of a plasmid. While this has worked as expected with many plasmid files that I got from a publication, it doesn't seem to work with the plasmids I constructed with UGENE. When I right-click and then go to Cloning -> Digest into Fragments, select the appropriate enzyme (Esp3I) and press OK, it doesn't perform the digest. In the log, there is this error message:

[ERROR][13:43] Trying to recover from error: Unexpected query result code: 19 (constraint failed) at src/datatype/AnnotationGroup.cpp:127

I left the "Conserved Annotations" field empty. I renamed some annotations from the previous cloning step but even removing them doesn't help.

I would be really grateful if somebody could tell me what I am doing wrong. One example file containing the plasmid is attached to this post. My UGENE version is 1.31.0.


Thanks a lot,
Nikolas

Hello Nikolas!

We've fixed the issue. Could you please verify that everything works fine now? We may send you a snapshot UGENE version if you tell us your operating system.