Hello, Sergey,

Could you, please, provide the file you try to create a database with and describe the steps you do in UGENE. Because, from this point, it's rather difficult to figure the problem out - too few details.

Best regards,
Dmitrii Sukhomlinov,
The UGENE team.

Hello, Sergey,

I am really sorry for the delayed message. The database could be properly created in UGENE with the file you sent to me, I checked it. That means, that there is some local problem you have. Let's figure it out.

First of all, please, check that all BLAST tools are set correctly. Click "Setting" -> "Preferences" and go to the "External tools" tab in the appeared "Application settings" dialog. Now check, that there are valid paths to all "BLAST+" tools. If they are not you may either reset all rettings to default ("General" -> "Restart settings on the next run" -> restart UGENE - probably, it'll work) or set path to the "tools" folder manually (click the "..." button in the right-top corner of the "External tools" tab, go to the UGENE directory -> "tools" and click "Open"). Then try again. If it won't work, please, tell me again.