UGENE Forum - cloning tool improperly marks types of fragment ends

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UGENE Forum › General Category › Bugs and Issues › cloning tool improperly marks types of fragment ends

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cloning tool improperly marks types of fragment ends (Read 4152 times)

Nov 10^th, 2012 at 2:21am

Anthony Offline
YaBB Newbies

Posts: 4

Hello,

I am running the Windows 7 64 bit version 1.11.3. After digesting the provided plasmid sequence with BamHI and AgeI using the cloning tool, I then went back into the cloning tool to ligate it to another molecule. The 3' end of the large fragment is blunt, when it should not be because it was cut with BamHI. It is interesting though because the 5' end of the other fragment has the proper overhang int the cloning window

Sincerely,
Anthony Bishop

pPD95_75_001.gb (51 KB | )

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Reply #1 - Nov 11^th, 2012 at 11:53pm

Konstantin Okonechnikov Offline
Global Moderator

Posts: 173

Hi Anthony,

thanks for bug report, the issue is fixed:
https://ugene.unipro.ru/tracker/browse/UGENE-1279

The fix will be included in 1.11.4.

In a couple of days (regular weekly build) it will be also available via development snapshot:
http://ugene.unipro.ru/snapshot.html

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