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digest into fragments in 1.11.1 works wrong for blunt end enzymes (Read 5192 times)
Sep 21st, 2012 at 3:27pm

randolf   Offline
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Hello,
When I do "digestion into fragments" of a plasmid with blunt end restriction enzymes, like EcoRV or SmaI, the fragments created have wrong properties. (and therefore the cloning process afterwards doesn't work). In the annotation manager, the 3'type and 5'type are "sticky" instead of "blunt" and 3'strand and 5'strand are both "rev-compI".

I hope this can be fixed. The cloning with sticky-end enzymes works great, thanks a lot for the program!
 
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Reply #1 - Sep 21st, 2012 at 3:30pm

randolf   Offline
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by the way, I use the 1.11.1 version for linux, 64bit
 
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Reply #2 - Sep 24th, 2012 at 2:57am

Konstantin Okonechnikov   Offline
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Hi! Tnx for report, the bug is reproduced and will be fixed ASAP.
Corresponding bug tracker entry:
https://ugene.unipro.ru/tracker/browse/UGENE-1202
 
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