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General Category >> Help and How-to >> Sanger to BLASTn analysis https://forum.ugene.net/forum/YaBB.pl?num=1569723195 Message started by bughunter on Sep 29th, 2019 at 9:13am |
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Title: Sanger to BLASTn analysis Post by bughunter on Sep 29th, 2019 at 9:13am
Hello,
I use 16S and ITS Sanger sequencing to identify organisms but I can't seem to find/create what I thought would be a pretty simple workflow. I'm not aligning to a known sequence as the entire point is to identify "unknown" organisms. My ideal workflow would be: Read Sequences > Sequence Quality Trimmer > CAP3 Assembly > BLASTn search It doesn't seem possible to connect the trim and assembly step, however (the tool for assembly only recognizes output from Read Sequences on my end). Does anyone have suggestions for creating this workflow? |
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Title: Re: Sanger to BLASTn analysis Post by Olga Golosova on Sep 30th, 2019 at 2:30pm
Thank you for your feedback!
Indeed, it's better to modify the CAP3 element, so that it supports the described scenario. I've created an issue about that in out tracker: https://ugene.dev/tracker/browse/UGENE-6607. |
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Title: Re: Sanger to BLASTn analysis Post by bughunter on Sep 30th, 2019 at 7:00pm
Thank you Olga! Much appreciated.
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