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General Category >> Help and How-to >> RTPCR Primer Design https://forum.ugene.net/forum/YaBB.pl?num=1407979136 Message started by lbroetto on Aug 14th, 2014 at 8:18am |
Title: RTPCR Primer Design Post by lbroetto on Aug 14th, 2014 at 8:18am
How can I insert the right Exon annotation name in order to use properly the new RTPCR Primer Design function on Primer3? Any exon information I have putted on this field result in fail.
Thanks. |
Title: Re: RTPCR Primer Design Post by Olga Golosova on Aug 14th, 2014 at 11:33am
To use the feature you should have annotations named "exon" on your input sequence.
An example of a file annotated in such way is provided in the documentation (GenBank accession ID: NM_001135099.1). Alternatively, you can also use another name, but you should then input it in the dialog: Quote:
The link to the documentation: https://ugene.unipro.ru/wiki/display/UUOUM/RTPCR+Primer+Design |
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