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General Category >> Help and How-to >> How to do reverse translation? https://forum.ugene.net/forum/YaBB.pl?num=1350933767 Message started by Malvolio on Oct 23rd, 2012 at 2:22am |
Title: How to do reverse translation? Post by Malvolio on Oct 23rd, 2012 at 2:22am
Hello!
Unipro Ugene is an excellent programme and I really enjoy using it. Unfortunately I couldn't find how to perform reverse translation in GUI mode, although this feature was announced long time ago. Googling "reverse translation" and "back translation" didn't yield anything. Please help! Thank you! PS currently I'm using version 1.9.3 from Ubuntu repository, but also have the latest one at hand. |
Title: Re: How to do reverse translation? Post by Olga Golosova on Oct 23rd, 2012 at 6:46pm
In the latest UGENE version 1.11.2 to make a reverse complement sequence in the Sequence View, one can use "Edit sequence > Reverse complement sequence" item in the context menu.
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Title: Re: How to do reverse translation? Post by Malvolio on Oct 24th, 2012 at 4:21am
Thank you for a rapid reply! But that's not what I meant... Sorry if I didn't state my problem clear enough!
Under reverse or back translation I meant taking a protein sequence and turning it to a nucleotide sequence using known codon usage tables. For this purpose I usually use this programme rev_trans from bioinformatics[dot]org, but it would be nice if I could do it in Ugene interface and the question is if it's possible in current version. Citing the Ugene manpage: Quote:
Thanks! |
Title: Re: How to do reverse translation? Post by Malvolio on Oct 24th, 2012 at 5:04am
By trial and error I figured out how to do back translation in version 1.11.2. Just press "export sequence" for desired amino acid sequence, check "translate back to nucleic acid alphabet" and select the desired organism. Ugene is great!
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