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https://forum.ugene.net/forum/YaBB.pl General Category >> Bugs and Issues >> digest into fragments in 1.11.1 works wrong for blunt end enzymes https://forum.ugene.net/forum/YaBB.pl?num=1348216077 Message started by randolf on Sep 21^st, 2012 at 3:27pm

Title: digest into fragments in 1.11.1 works wrong for blunt end enzymes
Post by randolf on Sep 21^st, 2012 at 3:27pm

Hello,
When I do "digestion into fragments" of a plasmid with blunt end restriction enzymes, like EcoRV or SmaI, the fragments created have wrong properties. (and therefore the cloning process afterwards doesn't work). In the annotation manager, the 3'type and 5'type are "sticky" instead of "blunt" and 3'strand and 5'strand are both "rev-compI".

I hope this can be fixed. The cloning with sticky-end enzymes works great, thanks a lot for the program!

Title: Re: digest into fragments in 1.11.1 works wrong for blunt end enzymes
Post by randolf on Sep 21^st, 2012 at 3:30pm

by the way, I use the 1.11.1 version for linux, 64bit

Title: Re: digest into fragments in 1.11.1 works wrong for blunt end enzymes
Post by Konstantin Okonechnikov on Sep 24^th, 2012 at 2:57am

Hi! Tnx for report, the bug is reproduced and will be fixed ASAP.
Corresponding bug tracker entry:
https://ugene.unipro.ru/tracker/browse/UGENE-1202