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General Category >> Help and How-to >> "Digest into Fragments" and circular molecules https://forum.ugene.net/forum/YaBB.pl?num=1344353059 Message started by hatta0 on Aug 7th, 2012 at 10:24pm |
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Title: "Digest into Fragments" and circular molecules Post by hatta0 on Aug 7th, 2012 at 10:24pm
How do I get "Digest into Fragments" to consider my plasmid to be circular? The only place I've found to set the molecule to circular
is in "Find Restriction Sites" but that doesn't seem to keep after hitting OK. Enabling the circular view doesn't help either. Is there a way to do this? |
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Title: Re: "Digest into Fragments" and circular molecules Post by Konstantin Okonechnikov on Aug 16th, 2012 at 4:16am
This feature is missing, it will be added in next bug-fix version:
https://ugene.unipro.ru/tracker/browse/UGENE-1156 For now it's possible to do the following trick: 1) From context menu in Sequence View select Cloning->Create Fragment. In appeared dialog leave default settings and click OK. This will result in creating a DNA fragment annotation. 2) From context menu in Sequence View select Cloning->Construct molecule. In appeared dialog add the created fragment to molecule fragment list and set option Make circular selected. Click OK. This will result in creating a circular sequence, which equals to the source. |
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Title: Re: "Digest into Fragments" and circular molecules Post by Konstantin Okonechnikov on Oct 10th, 2012 at 5:00am
Feature is available in 1.11.2
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