Dear UGENE developers,

I am facing some problems running a local BLAST+. The problem is the following:

I tried to run BLAST+ from UGENE the first time and went ok.
Next I tried to run BLAST from BioEdit and it also went fine.
When I tried to run BLAST+ from UGENE the following error message appeared:
[ERROR][16:08] Critical: (110.6) CNcbiRegistry: Syntax error in system-wide configuration file: NCBI C++ Exception:
[ERROR][16:08] "..\..\..\..\..\src\corelib\ncbireg.cpp", line 659: Error: ncbi::IRWRegistry::x_Read() - Badly placed '\' in the registry value: 'ROOT=C:\nASNLOAD=C:\BioEdit\tables\nDATA=C:\BioEdit\tables\' (m_Pos = 4)
[ERROR][16:08] Error: NCBI C++ Exception:
[ERROR][16:08] "..\..\..\..\..\src\corelib\ncbireg.cpp", line 659: Error: ncbi::IRWRegistry::x_Read() - Badly placed '\' in the registry value: 'ROOT=C:\nASNLOAD=C:\BioEdit\tables\nDATA=C:\BioEdit\tables\' (m_Pos = 4)
[ERROR][16:08] Task {Run NCBI BlastAll multitask} finished with error: "..\..\..\..\..\src\corelib\ncbireg.cpp", line 659: Error: ncbi::IRWRegistry::x_Read() - Badly placed '\' in the registry value: 'ROOT=C:\nASNLOAD=C:\BioEdit\tables\nDATA=C:\BioEdit\tables\' (m_Pos = 4)

I already uninstall both Bioedit and UGENE and install only UGENE but the problem still persists.

I also downloaded the external tools for Windows 64x but the problem still persists.

Can anybody help me? I suspect this should be hard to recover...

Best regards
PAL  

Dear PAL,

It seems that the problem occurred because BioEdit wrote some lines into the "ncbi.ini" file.

UGENE doesn't need this file to run BLAST+. So, as a temporary solution you can try to search the "ncbi.ini" file in your file system and delete it.

However, we're going to fix this issue in future UGENE versions: https://ugene.unipro.ru/tracker/browse/UGENE-791

Kind regards,
Olga Golosova
Unipro UGENE Team

Looks like a bug in the environment variables.
Can you send the values ​​of environment variables?
To do this, run cmd.exe and in the console, run the following commands

Code:

> c: > set > env.txt

And send us the contents of the created file C:\env.txt or the file itself.

Best regards
Denis