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General Category >> Help and How-to >> Phylognetic tree ugene plugin
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Message started by Pluszzz on Mar 11th, 2011 at 11:30pm

Title: Phylognetic tree ugene plugin
Post by Pluszzz on Mar 11th, 2011 at 11:30pm
How I can do phylogenetic tree using ugene?
First of all, on the web http://ugene.unipro.ru/plugins.html I can't see phylip plugin to downolad, second- when I go to Settings-->Plugins, plugin Phylip is on, so maybe it is already instaled? But I can't find it!

Title: Re: Phylognetic tree ugene plugin
Post by Agu on Mar 12th, 2011 at 12:29am
Just make the alignment I you will see the icon of the tree with a bird on top next to the camera icon.
Also, you might want to have a look to this video with the basics of alignments:
http://www.youtube.com/watch?v=lD2JqfxKlk0

All episodes here:
http://genome.unipro.ru/podcast_archive.html
tree.PNG (18 KB | )

Title: Re: Phylognetic tree ugene plugin
Post by Pluszzz on Mar 12th, 2011 at 12:43am
The problem is that I don't have this icon. I have ugene 1.9.1., from http://ugene.unipro.ru/download.html.
sd.JPG (15 KB | )

Title: Re: Phylognetic tree ugene plugin
Post by Agu on Mar 12th, 2011 at 12:49am
Did you make the alignment first? In the image you sent I can see a normal molecule opened and not an alignment.
The Icon only appears when it can be used (with an alignment).

Title: Re: Phylognetic tree ugene plugin
Post by Agu on Mar 12th, 2011 at 1:01am
One way to start the alignment is exporting a complete sequence as alignment (export_alignment.png) or features from one or several sequences (same feature from two different sequences in Features_alignment.png). Then you can add more sequences, delete, align, etc (check the options with the right button).
export_alignment.png (83 KB | )
Features_alignment.png (170 KB | )

Title: Re: Phylognetic tree ugene plugin
Post by Pluszzz on Mar 12th, 2011 at 1:09am
Thanks a lot. I found it. BTW- is there some special tool to find conserved domains within ugene?

Title: Re: Phylognetic tree ugene plugin
Post by Agu on Mar 12th, 2011 at 1:25am
After selecting the region (or nothing so it will use the whole sequence), press the icon for "Query Remote Database" (1), then instead of blastn (2), choose ccd (conserved domain database). The advantage of doing that from ugene, is that it will be automatically annotated.
If you are searching for a special pattern, you can do a manual search using Find pattern (3) or the same but using Smith-Waterman algorithm (4). You can also search for transcription factors binding sites (5).
You can also automate this tasks and preform the same over several sequences at the same time using this tools inside the Workflow designer (no number..., go to tools).
cdd.png (68 KB | )

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