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Message started by matt on May 31st, 2010 at 8:51pm

Title: Feature Database / Scan for feature
Post by matt on May 31st, 2010 at 8:51pm
i searched - but couldnīt find.

Letīs say i am always working with plasmid or DNA which contains several primer binding sites, which i use regulary (for example a primer binding in a selection cassette).
Is it possible to load a dna sequence and scan it for particular features?
Or even create and extend that feature list (like a group with common feature, a group for features appearing in lab-project x):?

is this possible somehow or do i have to enter new features for each new project???

Title: Re: Feature Database / Scan for feature
Post by Konstantin Okonechnikov on Jun 2nd, 2010 at 12:03pm
matt,
I suppose that this problem could be solved with workflow designer, but need some more details.
could you please clarify your request a little bit?

what particular steps do you perform in your task? (like search for DNA pattern, primer etc.)
could you give an example of query "feature-list" to be executed on a sequence?

Title: Re: Feature Database / Scan for feature
Post by Sebastian on Jun 8th, 2010 at 9:56pm
Hi,

I guess matt means auto-annotation of DNA-features (CDS, Promoters, Tags, ...)?
I'm also looking for a way to auto-annotate my Sequences. In detail: scan a dna/aa sequence for known dna and aa patterns (from an annotation db or file) and create annotations for the input sequence.

Should do the same like "Scan for features" in Serial Cloner or "Auto-annotate sequence" in GENtle.

Title: Re: Feature Database / Scan for feature
Post by matt on Jun 18th, 2010 at 3:11pm
exactely.
My departement is working with gentle and pdraw32- but i somehow try ugene for my stuff first.
Just as Sebastian said - i meant the auto annotation of DNA features of the Sequence youīre working with.
So for example - if you start working with a quite common vector - you can add features - like promotor regions, selection cassettes, primers etc. once - and than simply "scan" new added sequences for those features instead of annotating each one for itself.

Another example - describing my case (means: beside a feature section for common annotation features (like in the example above - stuff that is used in several labs) it would be great to introduce my own features - which fit to my current project)
In my case it is for a common cloning project where:
1. i start with the gene of interest
2. use the cDNA for creating a vector including parts of it
3. introduce a selection cassette and amplify
4. use the amplified vector for transformation
So on each step several features are the same - so it would make things easier to enter annotations to a list or something similar once - and then simply "scan" your sequence of choice for those.

Hope you got what i mean.
Guess most wishes here lead to features similar to gentle, pdraw, serial cloner, ape and in special probably vectorNti  ;)

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